Microplastics have been considered as an emerging pollutant in the aquatic environment. However, research about microplastic  ollution in inland freshwaters of China is insufficient. The present study investigated the levels of microplastics in surface water of 20 urban lakes and urban reaches of the Hanjiang River and Yangtze River of Wuhan, the largest city in central China.  icroplastic concentrations ranged from 1660.0 ± 639.1 to 8925 ± 1591 n/m3 for the studied waters, with the highest concentration found in Bei Lake. Microplastic abundance in lakes varied markedly in space, and negatively correlated with the distance from the city center (p b 0.001), which confirmed the important role of anthropogenic factors in microplastic distribution. Urban reaches of the Hanjiang River and Yangtze River were found to have relatively lower levels of microplastics than most of the studied lakes. The major type of microplastics among the studied waters was colored plastic, with fiber being the most frequent shape. More than 80% of microplastics in number had a size of b2mm. Polyethylene terephthalate and polypropylene were the dominant polymer-types of  microplastics analyzed. This study provided important reference for better understanding microplastic levels in inland freshwaters.