Plants frequently encounter a combination of abiotic stresses in their natural habitats. Abiotic stresses, including drought, salt, cold, heat, and heavy metal etc., modulate phytohormone metabolism and enhance expression level of transcription factors which activate stress responsive genes. During plant stress response, reactive oxygen species (ROS) act as important molecules and play pivotal roles in activating downstream metabolic pathways. In this Research Topic, we collected 25 manuscripts related to ROS and redox regulation in plant responses to abiotic stress, including reviews of the role of ROS in plant abiotic stress responses and articles related to gene function analysis, genome-wide gene expression and transcriptomic analysis, and interaction analysis between ROS and phytohormones.