Actinidia latifolia, known for its high vitamin C content in fruits, is widely distributed in the subtropical and tropical regions of eastern and southern Asia. In this study, we sequenced the complete chloroplast (cp) genome sequence ofA.latifoliato investigate its phylogenetic position in the gunusActinidia. The complete cp genome sequence is 156,873 bp in length with 37.15% overall GC content and characterized by a typical quadripartite structure comprising one pair of inverted repeats (23,377 bp) separated by a small single-copy region (21,453 bp) and a large single-copy region (88,666 bp). The cp genome contains 132 unique genes, including 83 protein coding genes, 41 tRNA genes, and 8 rRNA genes. Phylogenetic analysis highly supported thatA. latifoliaevolutionarily close toA. eriantha.