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  您现在的位置:首页 > 科研成果 > 论文
 
论文编号:
作者:
作者: Wang, Zupeng; Liu, Xiaoying; Xie, Xiaodong; Deng, Lei; Zheng, Hao; Pan, Hui; Li, Dawei; Li, Li; Zhong, Caihong*
作者所在部门:
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通讯作者:
通讯作者: Zhong, Caihong*
刊物名称:
刊物名称: INTERNATIONALJOURNAL OF MOLECULAR SCIENCES
论文题目:
论文题目: ABE8e with Polycistronic tRNA-gRNA Expression Cassette Sig-Nificantly Improves Adenine Base Editing Efficiency in Nicotiana benthamiana
摘要:  
摘要:
 Adenine base editor containing TadA8e (ABE8e) has been reported in rice. However, the application of ABE8e in other plant species has not been described, and the comparison between ABE8e and ABE7.10, which is widely used in plants, has also been poorly studied. Here, we developed the ABE8e with the polycistronic tRNA-gRNA expression cassette (PTG-ABE8e) and PTG-ABE7.10 and compared their A-to-G editing efficiencies using both transient and stable transformation in the allotetraploid Nicotiana benthamiana. We found that the editing efficiency of PTG-ABE8e was significantly higher than that of PTG-ABE7.10, indicating that ABE8e was more efficient for A-to-G conversion in N. benthamiana. We further optimized the ABE8e editing efficiency by changing the sgRNA expression cassette and demonstrated that both PTG and single transcript unit (STU) enhanced ABE8e efficiency for A-to-G conversion in N. benthamiana. We also estimated the potential off-target effect of PTG-ABE8e at potential off-targeting sites predicted using an online tool in transgenic plants, and no off-target editing event was found for potential off-targeting sites selected, indicating that ABE8e could specifically facilitate A-to-G conversion. Our results showed that ABE8e with PTG structure was more suitable for A-to-G conversion in N. benthamiana and provided valuable clues for optimizing ABE tools in other plants
年: 2021
卷: 22
期: 11
页: 5663
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备注: DOI:10.3390/ijms22115663

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